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dna band fluorescent intensities  (Thermo Fisher)


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    Thermo Fisher dna band fluorescent intensities
    PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
    Dna Band Fluorescent Intensities, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+band+fluorescent+intensities/DNA/pmc00196609-422-25-30
    Average 99 stars, based on 1 article reviews
    dna band fluorescent intensities - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Reduction of Stability of Arabidopsis Genomic and Transgenic DNA-Repeat Sequences (Microsatellites) by Inactivation of AtMSH2 Mismatch-Repair Function 1 "

    Article Title: Reduction of Stability of Arabidopsis Genomic and Transgenic DNA-Repeat Sequences (Microsatellites) by Inactivation of AtMSH2 Mismatch-Repair Function 1

    Journal:

    doi: 10.1104/pp.103.023952

    PCR amplification of DNA containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures by capillary electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
    Figure Legend Snippet: PCR amplification of DNA containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures by capillary electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.

    Techniques Used: Amplification, Electrophoresis, Labeling, Staining

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Reduction of Stability of Arabidopsis Genomic and Transgenic DNA-Repeat Sequences (Microsatellites) by Inactivation of AtMSH2 Mismatch-Repair Function
    Article Snippet: .. Up to nine locus-specific PCR reaction products were diluted as empirically found appropriate for subsequent analysis, mixed, and analyzed by capillary electrophoresis and measurement of DNA-band fluorescent intensities, using the ABI Prism 3100 Genetic Analyzer and associated software (Applied Biosystems, Palo Alto, CA). .. Product sizes were precisely and reproducibly assigned by comparison with an internal lane standard using Genes-can software (GS400HD Rox, Applied Biosystems), although absolute size measurements differed from genome-predicted sizes.

    Electrophoresis:

    Article Title: Reduction of Stability of Arabidopsis Genomic and Transgenic DNA-Repeat Sequences (Microsatellites) by Inactivation of AtMSH2 Mismatch-Repair Function
    Article Snippet: .. Up to nine locus-specific PCR reaction products were diluted as empirically found appropriate for subsequent analysis, mixed, and analyzed by capillary electrophoresis and measurement of DNA-band fluorescent intensities, using the ABI Prism 3100 Genetic Analyzer and associated software (Applied Biosystems, Palo Alto, CA). .. Product sizes were precisely and reproducibly assigned by comparison with an internal lane standard using Genes-can software (GS400HD Rox, Applied Biosystems), although absolute size measurements differed from genome-predicted sizes.

    Software:

    Article Title: Reduction of Stability of Arabidopsis Genomic and Transgenic DNA-Repeat Sequences (Microsatellites) by Inactivation of AtMSH2 Mismatch-Repair Function
    Article Snippet: .. Up to nine locus-specific PCR reaction products were diluted as empirically found appropriate for subsequent analysis, mixed, and analyzed by capillary electrophoresis and measurement of DNA-band fluorescent intensities, using the ABI Prism 3100 Genetic Analyzer and associated software (Applied Biosystems, Palo Alto, CA). .. Product sizes were precisely and reproducibly assigned by comparison with an internal lane standard using Genes-can software (GS400HD Rox, Applied Biosystems), although absolute size measurements differed from genome-predicted sizes.



    Similar Products

    dna band fluorescent intensities  (Thermo Fisher)
    99
    Thermo Fisher dna band fluorescent intensities
    PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
    Dna Band Fluorescent Intensities, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+band+fluorescent+intensities/DNA/pmc00196609-422-25-30
    Average 99 stars, based on 1 article reviews
    dna band fluorescent intensities - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    PCR amplification of DNA containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures by capillary electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.

    Journal:

    Article Title: Reduction of Stability of Arabidopsis Genomic and Transgenic DNA-Repeat Sequences (Microsatellites) by Inactivation of AtMSH2 Mismatch-Repair Function 1

    doi: 10.1104/pp.103.023952

    Figure Lengend Snippet: PCR amplification of DNA containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures by capillary electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.

    Article Snippet: Up to nine locus-specific PCR reaction products were diluted as empirically found appropriate for subsequent analysis, mixed, and analyzed by capillary electrophoresis and measurement of DNA-band fluorescent intensities, using the ABI Prism 3100 Genetic Analyzer and associated software (Applied Biosystems, Palo Alto, CA).

    Techniques: Amplification, Electrophoresis, Labeling, Staining